Sensitive enzymatic quantification of 5-hydroxymethylcytosine in genomic DNA
نویسندگان
چکیده
The recent discovery of genomic 5-hydroxymethylcytosine (hmC) and mutations affecting the respective Tet hydroxylases in leukemia raises fundamental questions about this epigenetic modification. We present a sensitive method for fast quantification of genomic hmC based on specific transfer of radiolabeled glucose to hmC by a purified glucosyltransferase. We determined hmC levels in various adult tissues and differentiating embryonic stem cells and show a correlation with differential expression of tet genes.
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عنوان ژورنال:
دوره 38 شماره
صفحات -
تاریخ انتشار 2010